RNA

Part:BBa_K2186000

Designed by: Nithin Saripalli   Group: iGEM16_CAPS_Kansas   (2016-10-14)


gRNA targeting TolC in E. Coli BL21(DE3)

This part is a gRNA that targets the TolC gene in E. Coli BL21(DE3). This is to be used in a CRIPSR plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 32
    Illegal BsaI.rc site found at 6


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Categories
Parameters
None