Translational_Unit

Part:BBa_K2182003

Designed by: David Rosenberg   Group: iGEM16_Northeastern   (2016-10-14)


Translational Unit for His-tagged NOX

This is the translational unit for the expression of poly-histidine tagged NOX, BBa_K2182001. It drives the expression of tagged NADH Oxidase, which catalyzes the reaction NADH + O2 --> NAD+ + H2O. The polyhistidine tag can be used to isolate the purified protein using ELISA, although this has not been successfully verified.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1341
    Illegal XbaI site found at 2874
    Illegal PstI site found at 534
    Illegal PstI site found at 2067
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 48
    Illegal NheI site found at 71
    Illegal NheI site found at 849
    Illegal NheI site found at 2382
    Illegal PstI site found at 534
    Illegal PstI site found at 2067
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 494
    Illegal BamHI site found at 2027
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1341
    Illegal XbaI site found at 2874
    Illegal PstI site found at 534
    Illegal PstI site found at 2067
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1341
    Illegal XbaI site found at 2874
    Illegal PstI site found at 534
    Illegal PstI site found at 2067
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None