Part:BBa_K218025

autoinducer inactivation enzyme (aiiA) and Double terminators

The RBS-autoinducer inactivation enzyme-double terminators is a translational part cloned in the AHL-degrading response circuit.

Usage and Biology

This biological fragment is constructed from two previously Biobricked parts. The The autoinducer inactivation gene (aiiA) gene is originally isolated from Bacillus species, 240B1 strain. This gene encodes an enzyme which catalyzes the degradation of N-acyl-homoserine lactones (AHL), thereby disrupting Quorum Sensing. The aiiA gene was submitted to the registry in 2004 (Part:BBa_C0160). The double terminators (Part: BBa_B0015) follows this gene in the construct. The part is also available with a Ribosome Binding Site leading the construct (BBa_K218026).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 99
  
• 1000
  COMPATIBLE WITH RFC[1000]

Source

The source of B0015 and C0160 is the Registry of Standard Biological Parts.

Reference

Miller, M. B. & Bassler, B. L. 2001. Quorum sensing in bacteria. Annu Rev Microbiol 55:165-199