Coding

Part:BBa_K2180012

Designed by: Sofiane Safi-Stibler   Group: iGEM16_UPMC-Paris   (2016-08-22)


Promoter PlepA +FUR regulatory sequence + RBS + LacI-LVA + Terminator + Pigment + RBS + Operateur La

This biobrick allow the production of the blue pigment in the presence of iron.
This is the assembly of our parts BBa_K2180002, BBa_K2180006 and BBa_K2180008


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1360
    Illegal NheI site found at 2460
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 377
    Illegal AgeI site found at 1165
  • 1000
    COMPATIBLE WITH RFC[1000]


PS : On the wiki the BBa_K2180012 and BBa_K2180018 are interchanged

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Categories
Parameters
None