Regulatory

Part:BBa_K2180010

Designed by: Sofiane Safi-Stibler   Group: iGEM16_UPMC-Paris   (2016-08-21)


Cadmium Operator - MntR Binding Site

Biobrick based on a previous biobrick made by the iGEM09_Cornell Team (2009-08-10) :
http://partsregistry.org/Part:BBa_K285103:Design
We added two restriction sites: SacII before and PacI after the regulatory region in order to use it in our project. They allow us to replace a regulatory region with another regulatory region. This Region allow MntR to bind to DNA and repress transcription. In the presence of Cadmium transcription is derepressed because of the fixation of cadmium to MntR.
Other metallic ions may bind to MntR.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None