Part:BBa_K2180006
PLacO - Promoter LacO for B. subtilis
The part is made of a previous IGEM part made by the team iGEM12_LMU-Munich (2012-07-16) :
https://parts.igem.org/Part:BBa_K823002
We add two LacI binding sites (Gilbert and Maxam, PNAS, 1973)
This part is made of PlepA promoter, two LacI binding sites and a RBS. There is a PacI restriction site before LacI operator binding sites and a SacII restriction site after them. They allow us to replace the LacI binding sites by other operating sequences. A SalI restriction sites is presents before the PlepA sequence and a NdeI restriction site after the RBS that may be used to insert the whole sequence. We use this part in our project to promote the pigment expression under the control of LacI.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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