Regulatory

Part:BBa_K2180002

Designed by: Sofiane Safi-Stibler   Group: iGEM16_UPMC-Paris   (2016-08-01)


Fructose Operator - FruR Binding Site

The sequence of the regulatory region comes from this site
We added two restriction sites: SacII before and PacI after the regulatory region in order to use it in our project.
This restriction sites allow us to replace a regulatory region with another regulatory region.
The regulatory region is composed by two FruR binding sites wich are derepressed in the presence of fructose.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None