Reporter

Part:BBa_K2176000

Designed by: Miranda Halle   Group: iGEM16_UChicago   (2016-07-25)


yeGFP Reporter for LexA

This part contains a yeast-optimized GFP (yeGFP) coding sequence, flanked by an ADH1 terminator and a Cyc1 promoter. This promoter is under the control of a LexA operator, which only activates the promoter in the presence of the LexA protein. Thus, cells containing this construct will express GFP only in the presence of LexA.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 6
    Illegal suffix found in sequence at 1870
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal SpeI site found at 1871
    Illegal PstI site found at 1885
    Illegal NotI site found at 12
    Illegal NotI site found at 1878
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal XhoI site found at 176
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 6
    Illegal suffix found in sequence at 1871
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 6
    Illegal XbaI site found at 21
    Illegal SpeI site found at 1871
    Illegal PstI site found at 1885
    Illegal AgeI site found at 1865
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1074


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Categories
Parameters
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