Composite

Part:BBa_K2173004

Designed by: Chandel Angad Singh   Group: iGEM16_IIT_Delhi   (2016-10-21)


LuxI_LuxR_lambda-CI

luxI with LVA degradation tag expressed under Plux promoter and a double terminator + LuxR expressed under constitutive promoter J23100 and double terminator + heat sensitive C1-lambda under constitutive promoter and a double terminator

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 879
    Illegal NheI site found at 902
    Illegal NheI site found at 1869
    Illegal NheI site found at 1892
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 721
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None