Intermediate

Part:BBa_K2172008

Designed by: Bowen Xiao   Group: iGEM16_CIEI-BJ   (2016-10-14)


GST-Thrombin Protease-SmCPS1-TEV-GFP

This part is a gene circuit that can be used to produce the enzyme SmCPS1. SmCPS1 is a key enzyme on the biosynthesis pathway of tanshinone (a compound used to treat cardiovascular diseases). The tac promoter, a strong hybrid promoter, is used to control and increase the expression levels of the target gene (SmCPS1), and is used in the expression of recombinant proteins, produced from the combination of promoters from the trp and lac operons. The GST label is used to purify the recombinant protein. The GFP is used to measure the expression level of the part.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2580
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1341
    Illegal BamHI site found at 673
    Illegal BamHI site found at 939
    Illegal XhoI site found at 1075
    Illegal XhoI site found at 1099
    Illegal XhoI site found at 2392
    Illegal XhoI site found at 2602
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 744
    Illegal NgoMIV site found at 2161
    Illegal NgoMIV site found at 2725
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 85


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