Intermediate

Part:BBa_K2172007

Designed by: Bowen Xiao   Group: iGEM16_CIEI-BJ   (2016-10-14)


GST-Thrombin Protease-TEV-GFP

This part contains a GST label, a thrombin and a TEV protease cleavage site, a gene SmCPS1 obtained from Salvia miltiorrhiza, and a GFP detector. The GST label can be used to purify the target protein (in this case, SmCPS1). The two cleavage sites can be used to slice the target protein. GFP can be used to determine whether the part is expressed or not.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 673
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 85


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Categories
Parameters
None