Intermediate
Part:BBa_K2172007
Designed by: Bowen Xiao Group: iGEM16_CIEI-BJ (2016-10-14)
GST-Thrombin Protease-TEV-GFP
This part contains a GST label, a thrombin and a TEV protease cleavage site, a gene SmCPS1 obtained from Salvia miltiorrhiza, and a GFP detector. The GST label can be used to purify the target protein (in this case, SmCPS1). The two cleavage sites can be used to slice the target protein. GFP can be used to determine whether the part is expressed or not.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 673
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 85
[edit]
Categories
Parameters
None |