Coding

Part:BBa_K2171004

Designed by: Slavil Peykov, Desislava Popova   Group: iGEM16_Bulgaria   (2016-10-14)


SAHS2 - osmotic stress protein from tardigrade


Secretary abundant heat soluble protein 2 (SAHS2) - osmotic stress protein from tardigrade. This part is composed of K731500 (lacI-lacIq and a tac promoter) followed by a CDS of SAHS2 optimized for expression in E. coli. SAHS2 protein sequence was taken from UniProtKB: J7MAN2 (J7MAN2_9BILA). The eukaryotic secretion signal peptide (composed by 19 AA at N-terminus: MHRFVLALVVFAGAAIVWA) was removed and the remaining part ot the sequence was codon optimized for bacterial expression.


You can see how to create this part by yourself on page Design


Also see this part in our Wiki:

http://2016.igem.org/Team:Bulgaria/Part


References:

Ayami Yamaguchi, Sae Tanaka, Shiho Yamaguchi, Hirokazu Kuwahara, et al. (August 28, 2012); Two Novel Heat-Soluble Protein Families Abundantly Expressed in an Anhydrobiotic Tardigrade; PLoS ONE 7(8): e44209. doi:10.1371/journal.pone.0044209

Sae Tanaka, Junko Tanaka, Yoshihiro Miwa, Daiki D. Horikawa ,Toshiaki Katayama, et al. (February12,2015) Novel Mitochondria-Targeted Heat-Soluble Proteins Identified in the Anhydrobiotic Tardigrade Improve Osmotic Tolerance of Human Cells; PLoS ONE; doi:10.1371/journal.pone.0118272


Usage and Biology

This part was used by iGEM Bulgaria 2016 team in attempt to create genetic construct that could allow for the conversion of any microorganism into a resistant-towards-desiccation-and-temperature-stress type.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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