Part:BBa_K2171002

MASH - osmotic stress protein from tardigrade

Mitochondrial abundant heat soluble protein (MAHS) - osmotic stress protein from tardigrade with N-terminal signal peptide that targets the protein to the mitochondria. This part is composed of K731500 (lacI-lacIq and a tac promoter) followed by a CDS of MASH optimized for expression in E. coli. MAHS protein sequence was taken from UniProtKB: A0A0E4FH01 (A0A0E4FH01_9BILA). The mitochondrial targeting signal (composed by 30 AA at N-terminus: MSRYLLRDVQAVLRGVRKVAESSLKLETEK) was removed and the remaining part of the sequence was codon optimized for bacterial expression.

You can see how to create this part by yourself on page Design

Also see this part in our Wiki: http://2016.igem.org/Team:Bulgaria/Part

References:

Ayami Yamaguchi, Sae Tanaka, Shiho Yamaguchi, Hirokazu Kuwahara, et al. (August 28, 2012); Two Novel Heat-Soluble Protein Families Abundantly Expressed in an Anhydrobiotic Tardigrade; PLoS ONE 7(8): e44209. doi:10.1371/journal.pone.0044209

Sae Tanaka, Junko Tanaka, Yoshihiro Miwa, Daiki D. Horikawa ,Toshiaki Katayama, et al. (February12,2015) Novel Mitochondria-Targeted Heat-Soluble Proteins Identified in the Anhydrobiotic Tardigrade Improve Osmotic Tolerance of Human Cells; PLoS ONE; doi:10.1371/journal.pone.0118272

Usage and Biology

This part was used by iGEM Bulgaria 2016 team in attempt to create genetic construct that could allow for the conversion of any microorganism into a resistant-towards-desiccation-and-temperature-stress type.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]