Coding

Part:BBa_K2171001

Designed by: Slavil Peykov, Desislava Popova   Group: iGEM16_Bulgaria   (2016-10-14)


RvLEAM - osmotic stress protein from tardigrade


Late embryogenesis abundant protein, mitochondrial (RvLEAM) - osmotic stress protein from tardigrade. This part is composed of K731500 (lacI-lacIq and a tac promoter) followed by a CDS of RvLEAM optimized for expression in E. coli. RvLEAM protein sequence was taken from UniProtKB: A0A0E4AVP3 (A0A0E4AVP3_9BILA).


You can see how to create this part by yourself on page Design


Also see this part in our Wiki: http://2016.igem.org/Team:Bulgaria/Part


References:

Ayami Yamaguchi, Sae Tanaka, Shiho Yamaguchi, Hirokazu Kuwahara, et al. (August 28, 2012); Two Novel Heat-Soluble Protein Families Abundantly Expressed in an Anhydrobiotic Tardigrade; PLoS ONE 7(8): e44209. doi:10.1371/journal.pone.0044209

Sae Tanaka, Junko Tanaka, Yoshihiro Miwa, Daiki D. Horikawa ,Toshiaki Katayama, et al. (February12,2015) Novel Mitochondria-Targeted Heat-Soluble Proteins Identified in the Anhydrobiotic Tardigrade Improve Osmotic Tolerance of Human Cells; PLoS ONE; doi:10.1371/journal.pone.0118272


Usage and Biology

This part was used by iGEM Bulgaria 2016 team in attempt to create genetic construct that could allow for the conversion of any microorganism into a resistant-towards-desiccation-and-temperature-stress type.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None