Plasmid_Backbone

Part:BBa_K2170300

Designed by: Max Mustermann   Group: iGEM16_LMU-TUM_Munich   (2016-10-12)


Episomal stable eucaryotic expression vector (pDSG)

Plasmid for genomic integration in HEK293 cells

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 3189
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4092
    Illegal SpeI site found at 2
    Illegal SpeI site found at 3189
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 4098
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4092
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 4092
    Illegal suffix found at 2
    Illegal SpeI site found at 3189
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 4092
    Plasmid lacks a suffix.
    Illegal XbaI site found at 4107
    Illegal SpeI site found at 2
    Illegal SpeI site found at 3189
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 973


[edit]
Categories
Parameters
None