Part:BBa_K2169001
ZnO binding Curli protein
Curli subunit CsgA modified with a ZnO binding tag to enable binding of ZnO surfaces.
This device was overexpressed in NEB 5-alpha E. coli cells. The cells were incubated on a see-saw shaker to mediate biofilm formation. ZnOH was added in different varying concentrations for mineralization assays.
We noticed a distinct change in morphology of the resulting ZnO particles when compared to the curli-free reference. Furthermore, while without biofilm the particles were easily rinsed or wiped off, the biofilm was able to firmly affix the particles to the surface it was grown on.
Figure 1: ZnO nanoparticles grown in presence (left) and absence of curli fibers (CsgA-ZnOTag) (right) on a p-doped silica wafer
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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