Regulatory

Part:BBa_K216005

Designed by: Edinburgh iGEM 2009   Group: iGEM09_Edinburgh   (2009-09-25)

PyeaR promoter, responsive to nitrate, nitrite and nitric oxide

PyeaR promoter. This is the promoter of the Escherichia coli yeaR/yoaG operon (see Lin, H.-Y., Bledsoe, P.J., and Stewart, V. 2007. Activation of yeaR-yoaG operon transcription by the nitrate-responsive regulator NarL is independent of oxygen-responsive regulator Fnr in Escherichia coli K-12. J. Bacteriol. 189, 7539-7548). Unlike other E. coli promoters responding to nitrate and nitrite, this promoter is not repressed under aerobic conditions.

Usage and Biology

According to Lin et al (2007), this promoter is regulated mainly by phospho-NarL, although phospho-NarP can also activate it if NarL is not present. Repression of the promoter in the absence of nitrate/nitrite is mainly due to the repressor NsrR. Induction is higher under anaerobic conditions than under aerobic conditions, but strong induction still occurs under fully aerobic conditions; this is not true of other known E. coli promoters responsive to nitrate and nitrite. LacZ activities (Miller Units) were as follows:

  • anaerobic, complex medium, no induction: 5
  • anaerobic, complex medium, 40 mM nitrate: 460
  • anaerobic, complex medium, 5 mM nitrite: 97
  • anaerobic, minimal medium, no induction: 6
  • anaerobic, minimal medium, 40 mM nitrate: 3000
  • anaerobic, minimal medium, 5 mM nitrite: 680
  • aerobic, minimal medium, no induction: 2
  • aerobic, minimal medium, with 40 mM nitrate: 160

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//promoter
Parameters
None