Composite

Part:BBa_K2156017

Designed by: Carolina Sofia Gonzalez and Mikhail Lujan   Group: iGEM16_ColegioFDR_Peru   (2016-09-22)


Lux ABG with promoter and RBS

LuxABG is a combination of the separate LuxA, LuxB and LuxG parts, including the relevant Ribosome Binding Site (BBa_K1725301) and an Anderson Promoter (BBa_K823005). LuxAB is the required pair for an operational lux, with LuxG decreasing the energy required for bioluminescence. LuxC and Lux D are needed to synthesize and replenish the aldehyde substrate needed for bioluminescence. This was synthesized by IDT (Integrated DNA Technologies).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1085


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Categories
Parameters
None