Designed by: Yue Yang, Xin Hu, Bingzhao Zhuo Group: iGEM16_NWPU (2016-10-11)
Usage and Biology
We inserted a CcsDock (BBa_K2155004) behind our core enzyme TalB-Transaldolase B(Figure1). We ligated these two parts by using one-step cloning. With the dokerin, TalB can specially bind to scaffold protein and form a enzyme complex with BFD(Figure2). We have verified that the yeild of xylulose could be greatly improved when we use the scaffold protein to combine TalB and BFD.
Figure1. Dockerin is inserted behind our gene of interest.
Figure2. Enzymes(BFD and TalB) with dockerin bind to scaffold protein and form a enzyme complex.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21INCOMPATIBLE WITH RFCIllegal BamHI site found at 861
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC