Coding

Part:BBa_K215250

Designed by: Alex Leone   Group: iGEM09_Washington   (2009-09-30)

Lpp + 1tmr OmpA + GS Linker + TEV site + NheI Site

This is a fusion protein that displays your favorite gene inserted into the NheI site. A TEV site is placed before the NheI site to allow for easy removal of the protein of interest from the surface of the cells by treatment with TEV protease.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 211
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None