Part:BBa_K2152001
human Epidermal Growth Factor（hEGF） with FLAG-tag
Human EGF（epidermal growth factor）is a 6045-Da protein with 53 amino acid residues. A signal peptide phoA is added at the N-terminus to facilitate secretion. We engineered two peptide tags,a phoA signal peptide at the N-terminus and a FLAG tag at the C-terminus of hEGF protein. PhoA is a fragment of signal peptide that guides the protein to go across cytomembrane and do not decrease activity of the protein. FLAG-tag is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. hEGF is originally described as a growth factor to stimulate cell growth, proliferation and differentiation by binding to its receptor EGFR. In our project, we used hEGF as a therapeutic agent to treat Inflammatory bowel diseases.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 198
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Figure 1. Western Blot of EGF with FLAG-tag. The red rectangle marked area is our expressed recombinant EGF protein. The track on the left is the control group without IPTG induction.
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