Part:BBa_K2151102

J23106-RBS-epsilon

Epsilon with Anderson Promoter

Toxin-antitoxin (TA) systems are commonly used in nature by low-copy number plasmids to ensure retention in daughter cells. TA systems work by expression of two genes; one encoding a toxin and the other encoding an anti-toxin. The key feature of TA systems is that the toxin has a longer half-life than the antitoxin. In this way, if the plasmid is lost by daughter cells, the antitoxin with a shorter half-life will degrade/be degraded and the toxin will be free to either kill the cell or prevent growth. In the case of the epsilon-zeta protein-based TA system used on the plasmid pSM19035, epsilon is the antitoxin and zeta is the toxin.

Usage and Biology

This part contains the sequence for epsilon with its natural strength RBS without zeta, with an Anderson promoter ligated upstream. It could be used to test the impact of the antitoxin on the growth/viability/performance of the target organism. The sequence for epsilon has had its GC content shifted using codon changes while trying to not change the efficiency of translation.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]