Part:BBa_K2151010

p25-B0034-K592009: Blue chromoprotein under control of medium constitutive promoter

This part consists of amilCP, a blue chromoprotein, with a strong RBS under the control of p25, a medium constitutive bacterial promoter. Its intended use is to serve as a visual promoter-reporter construct in lactic acid bacteria, potentially as an alternative to mCherry which may take longer to become visible. p25 was initially identified in a promoter probe survey of the Streptococcus thermophilus genome, alongside weaker promoters p8 and p20.

We used p25 as a candidate promoter to drive gene expression in both Streptococcus thermophilus and E. coli. Quantification of p25 using GFP in E. coli revealed promoter strength on par with medium Anderson promoters (30.8% as strong as J23100, 43.7% as strong as J23101, 130% as strong as J23106).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]