Composite

Part:BBa_K2150127

Designed by: Zepeng Mu   Group: iGEM16_UCAS   (2016-10-19)

toxin 5694 composite

This basic part is a CDS for toxin 5694 in our project. In our final construct, this gene is controlled by aTc, and we characterized this part by measuring the growth curve of E. coli induced by graded aTc. During the experiment, the gene does not have a RBS upstream to it, because evening choosing the weakest RBS (BBa_B0033) make it unable for us to get the right colony. This also provides an opportunity for future teams to characterize or improve or use this part.

T--UCAS--5694.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None