Composite

Part:BBa_K2150113

Designed by: Zepeng Mu   Group: iGEM16_UCAS   (2016-10-13)

toxin ParE2 induced by aTc

Usage and Biology

This part is used to build a kill-switch.

Characterization

Growth Curve

We measured the growth curve of bacteria expressing toxin ParE2.

T--UCAS--6249t-atc.png

The growth curve of wild type E. coli harboring empty vector is shown below.

T--UCAS--RGP Ptet.png

This construction is also used to identify the interaction between toxins and antitoxins. See: BBa_K2150117


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 455
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None