Composite

Part:BBa_K2150111

Designed by: Zepeng Mu   Group: iGEM16_UCAS   (2016-10-13)

toxin ParE2 induced by IPTG

Usage and Biology

This part is used to build a kill-switch.

Characterization

Growth Curve

We measured the growth curve of bacteria expressing toxin ParE2.

T--UCAS--6249t-IPTG.png

The growth curve of wild type E. coli harboring empty vector is shown below.

T--UCAS--RGP Ptac.png

We also studied the interaction between ParE2 and its corresponding antitoxin ParD2. See: BBa_K2150117

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1848
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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