Generator

Part:BBa_K215011

Designed by: Jeff Nivala   Group: iGEM09_Washington   (2009-10-01)

K215090+K215002: OpdA with Secretion Signal and Streptavidin Binding Tags

This part is OpdA (BBa_K215090) with an N-terminal tag that binds to streptavidin and a C-terminal tag that signals for secretion to the extracellular space (when combined with proper secretion system BBa_K215107). Its expression is IPTG inducible.

Usage and Biology

This part (K215011) had not been tested upon submission, but its components have each been individually tested - The enzyme OpdA has been shown to function as predicted (K215091) and it is inserted into an expression vector that has also been shown to work (K215002).



Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 84
    Illegal suffix found in sequence at 1982
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 84
    Illegal SpeI site found at 1983
    Illegal PstI site found at 1997
    Illegal NotI site found at 90
    Illegal NotI site found at 1990
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 84
    Illegal XhoI site found at 1211
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 84
    Illegal suffix found in sequence at 1983
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 84
    Illegal XbaI site found at 99
    Illegal SpeI site found at 1983
    Illegal PstI site found at 1997
    Illegal NgoMIV site found at 308
    Illegal AgeI site found at 503
    Illegal AgeI site found at 842
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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