Part:BBa_K2150028

pTet+RBS+T7RNAP+Ter+pT7+RBS+tetX-GFP(fusion protein)+DT

In this part, T7 RNA polymerase(T7RNAP) is expressed from a tetracycline-inducible promoter pTet. T7RNAP can activate the T7 promoter, thus producing the fusion protein in large quantity.

Usage and Biology

We design this part to further enhance the degrading capacity of our bacteria. The amount of tetX is reflected by the fluorescent intensity, and the degradation of tetracycline is indicated by the survival and growth of the cell.

Characterization

As shown in Fig.1, compariing to BBa_K2150015, this part proforms better in the presence of tetR, at all Tc concentration applied. Bacteria carrying this part grow better, and produce a larger amount of degrading-enzyme (represented by fluorescence intensity) in all than the Bacteria carrying BBa_K2150015.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 3406
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 4703