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Part:BBa_K2150026

Designed by: Jianyi Huang   Group: iGEM16_UCAS   (2016-10-12)


pT7+pTet+RBS+tetX-GFP(fusion protein)+RBS+T7RNAP+DT

A positive feedback device to over-express the fusion protein of tetX and GFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 670
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1967


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