Part:BBa_K2150025
pCon+RBS+tetR+DT+pTet+RBS+tetX-GFP(fusion protein)+DT
In this part, a fusion protein(referred to as tetX-GFP) of tetX(a tetracycline-degrading enzyme) and GFP combined with a 3*GGGGS linker is used to report the expression level of tetX. The first promoter(BBa_J23119) is constitutively on and can express tetR, a protein that can repress the downstream promoter pTet in the absence of tetracycline(Tc). As a result, no fusion protein tetX-GFP is expressed and no fluorescence can be detected. However, in the presence of Tc, tetR is repressed and repression over pTet is removed. This leads to the expression of tetX-GFP, degrading tetracycline and emitting fluorescence at the same time. This part can report activity of tetX in the presence of Tc by emission of green fluorescence.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1533
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2830
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