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Part:BBa_K2150018

Designed by: Jianyi Huang   Group: iGEM16_UCAS   (2016-10-12)


pT7+pTet+RBS+GFP+DT

Two promoters are used, a constitutive promoter pTet and a T7RNAP specific promoter pT7. By testing the fluorescence intensity, we found that in the absence of T7 promoter, these two promoters exhibit higher efficiency than single pTet. With pT7, expression of GFP can be amplified while with pTet, expression of GFP can be repressed by tetR.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 752


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