Reporter

Part:BBa_K2150016

Designed by: Jianyi Huang   Group: iGEM16_UCAS   (2016-10-12)


pT7 GFP

T7 promoter and RBS and GFP, with double terminator.

Usage and Biology

We use this part to produce a high level of reporter protein GFP in our simulation system where T7 RNA polymerase is present. We observed a leakage of T7 promoter on high-copy plasmid pSB1C3.

PT7+GFP.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 690


[edit]
Categories
Parameters
None