Reporter

Part:BBa_K2150015

Designed by: Jianyi Huang   Group: iGEM16_UCAS   (2016-10-12)


pTet TetX-GFP(fusion protein)

This part is tetX-GFP with pTet promoter.

Usage and Biology

We use this part to create an antibiotic scavenger in the presence of tetR. The performance of this part was compared with BBa_K2150027 when tetR existed in the cell. The data showed BBa_K2150027 had a better capacity of degrading tetracycline than this part(see BBa_K21500028).

Characterization

This part showed a lower fluoresence intensity than BBa_2150014 at the same growth stage of E.coli (see BBa_K2150013).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 642
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1939


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Parameters
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