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Part:BBa_K2149020

Designed by: Aline Larissa Gonçalves   Group: iGEM16_USP-EEL-Brazil   (2016-10-19)


RFP with Lux D and an aldehyde decarbonylase

The Red Fluorescent Protein (RFP) is a gene reporter used to signalize the expression of Lux D, a transferase which is part of an operon (operon Lux C, E and D) involved in the biosynthesis of alkanes. After the end of gene Lux D there is an aldehyde decarbonylase that catalyzes the conversion of fatty aldehydes, produced by operon Lux, to alkanes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1897
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1285
    Illegal BamHI site found at 1678
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 555
    Illegal AgeI site found at 667
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1719


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Categories
Parameters
None