Composite

Part:BBa_K2149017

Designed by: Aline Larissa Gonçalves   Group: iGEM16_USP-EEL-Brazil   (2016-10-18)


Operon Lux C,E and D with an aldehyde decarbonylase

The operon Lux formed by the genes C, E and D are components of a fatty acid reductase system with reductase, synthetase and transferase activities, respectively. Using fatty acids as a substrate for alkanes biosynthesis, the Lux D gene provides a fatty acid substrate for the fatty acyl synthetase LuxE, that act cleaving activated fatty acids. The fatty acyl reductase subunit (LuxC) then uses the product of the LuxE to produce a fatty aldehyde, an aldehyde with a "fatty" aliphatic carbon chain attached that is typically eight carbon or more in length. After the production of fatty aldehyde occurs the action of the aldehyde decarbonylase, that catalyzes the conversion of fatty aldehydes to alkanes by the retirade of the aliphatic carbon chain.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2443
    Illegal NheI site found at 3827
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3215
    Illegal BamHI site found at 552
    Illegal BamHI site found at 2382
    Illegal BamHI site found at 3608
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 466
    Illegal AgeI site found at 2185
    Illegal AgeI site found at 2400
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3649
    Illegal SapI.rc site found at 2020


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Categories
Parameters
None