Translational_Unit

Part:BBa_K2149016

Designed by: André Tomas Vilela Hermann   Group: iGEM16_USP-EEL-Brazil   (2016-10-13)


luxE (Acyl-protein synthetase)

LuxE is an acyl-protein synthetase found in bioluminescent bacteria. LuxE catalyses the formation of an acyl-protein thiolester from a fatty acid and a protein. This is the second step in the bioluminescent fatty acid reduction system, which converts tetradecanoic acid to the aldehyde substrate of the luciferase-catalysed bioluminescence reaction [PMID: 8941351]. This enzyme takes the fatty acid substrate and catalyzes a reaction, forming a acyl-protein thioester product. Together with the LuxC gene, represents the main reaction of the fatty aldehyde synthesis, which can then be used to produce alkanes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 988
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 927
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 730
    Illegal AgeI site found at 945
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 565


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Categories
Parameters
None