Translational_Unit

Part:BBa_K2149001

Designed by: Aline Larissa Gonçalves   Group: iGEM16_USP-EEL-Brazil   (2016-10-13)


HPPD from Pseudomonas putida

This part coding a 4-hydroxyphenylpyruvate dioxygenase (HPPD) taken from Pseudomonas putida. This gene is involved in the pathway production of tocotrienol (vitamin E) and it is an oxygenase that catalyzes the second reaction in the catabolism of tyrosine - the conversion of 4-hydroxyphenylpyruvate into homogentisate (HGA). The HGA produced by 4-hydroxyphenylpyruvate dioxygenase that is one of many steps in break l-tyrosine into acetoacetate and fumarate. HPPD is part of a class of oxygenase enzymes that usually utilize α-ketoglutarate and diatomic oxygen to oxygenate or oxidize a target molecule. It is part of the cycle to create energy in almost aerobic organisms, but in eukaryotes, HPPD has a very important function that regulates blood tyrosine levels. Mutations in the N-terminal region of this protein cause the disease known as hawkinsinuria and HPPD can also be linked to one of the oldest known inherited metabolic disorders known as alkaptonuria. Plants utilize this enzyme to produce the cofactors plastoquinone and tocopherol which are essential for the plant to survive, this is why there are several HPPD inhibitor herbicides that block the activity of this enzyme and cause the plant's death.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 82
    Illegal PstI site found at 964
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 82
    Illegal PstI site found at 964
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 82
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 82
    Illegal PstI site found at 964
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 82
    Illegal PstI site found at 964
    Illegal NgoMIV site found at 381
    Illegal NgoMIV site found at 502
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 865


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Parameters
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