Coding

Part:BBa_K2148011

Designed by: Chern Juin Ong   Group: iGEM16_Cambridge-JIC   (2016-10-11)


aeBlue C.reinhardtii-CO Blue FP

This is a coding DNA sequence (CDS) for a blue fluorescent protein which is codon-optimised for Chlamydomonas reinhardtii chloroplast. It is coded as a CDS/CTAG Phytobrick.


Usage and Biology

This is a level-0 Phytobrick conforming to the Phytobrick standard. It is coded as a CDS/CTAG Phytobrick and can be used with other level-0 Phytobricks to create a transcriptional unit that expresses a blue fluorescent protein for Chlamydomonas reinhardtii chloroplast.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 225
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 225
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 225
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 225
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 225
  • 1000
    COMPATIBLE WITH RFC[1000]



Characterisation

References

[edit]
Categories
Parameters
None