DNA

Part:BBa_K2146006

Designed by: Zhixin Tan   Group: iGEM16_Emory   (2016-10-19)


pWH1266 ori-Ptac-lacOc-ampC ADC-33-T1-lacI-PT5-gusA-T2-pSB1C3

A. baylyi shuttle vector with cefotaxime resistance, containing reporter gene gusA which shows by its blue color when growing on media containing histochemical substrate X-gluc.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 561
    Illegal NheI site found at 1351
    Illegal NheI site found at 2689
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 368
    Illegal BamHI site found at 4371
    Illegal XhoI site found at 4218
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2454
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None