Reporter
GG100
Part:BBa_K2145125
Designed by: Katie Maruna Group: iGEM16_Alverno_CA (2016-10-19)
This part contains 2 fluorescent protein coding sites (RFP and GFP) with a spacer
This part contains two fluorescent protein coding sites (RFP and GFP) and was assembled using Golden Gate Assembly. In order to make the plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) along with a 500 base pair "spacer" part derived from different backbones, and a pSB1C3 backbone assembled to make the final part. It can be used to express both GFP and RFP proteins.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 825
Illegal AgeI site found at 937 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |