Deleted
Sample Discontinued
Experience: None
No Sequence
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Plasmid

Part:BBa_K2145006

Designed by: Laura Xinyu Chen   Group: iGEM16_Alverno_CA   (2016-10-14)


GFP/RFP reporter with spacer part (532bp)

This plasmid contains a GFP and an RFP reporter separated by a 532-bp spacer containing three gRNA binding sites for dCas. The reporter is designed to demonstrate the effect, if any, of binding a dCas "clamp" (or a random sequence in) between two genes (the idea being that such a clamp can limit propagation of supercoiling between the genes, functionally isolating them).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None