Deleted
Sample Discontinued
Experience: None
Not Used
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Reporter

Part:BBa_K2145003

Designed by: Melody Wu   Group: iGEM16_Alverno_CA   (2016-10-14)


GFP/RFP reporter with spacer (500bp)

This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1621
    Illegal AgeI site found at 1733
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 213


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Parameters
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