Deleted
Sample Discontinued
Experience: None
Not Used
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Reporter
GG97

Part:BBa_K2145002

Designed by: Melody Wu   Group: iGEM16_Alverno_CA   (2016-10-14)


GFP/RFP reporter with spacer (500bp)

This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) along with a "spacer" part (500bp) and pSB1C3 backbone assembled to make the final part. It can be used to express both GFP and RFP proteins.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 781
    Illegal AgeI site found at 893
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1801


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