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Coding

Part:BBa_K2144004

Designed by: Oskar hman   Group: iGEM16_Stockholm   (2016-10-12)


Coding sequence for Nuclease with His6 and LPXTG tag regulated by T7-promoter

Coding sequence for Nuclease from Staphylococcus Aureus, regulated by T7-promoter. Nuclease has the ability to cleave DNA. The inserted Linker Tag-sequence (BBa_K2144009) contains the gene encoding for His-tag which enables the use of IMAC to purify the combat protein. Also, it contains the LPTXG-tag. The LPXTG-tag enables the use of the "match making" enzyme sortase. The key feature of sortase is the specific conjugation reaction it carries out, where the enzyme recognizes a specific amino acid sequence, a so called sorting motif (LPXTG motif in the case of S.aureus) and conjugate this sequence with another unit carrying an oligo glycine motif. The Linker in the Linker tag sequence enables for proper folding of the protein.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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