Regulatory

Part:BBa_K2143000

Designed by: Bryan Pasqualucci   Group: iGEM16_SCSU-New_Haven   (2016-10-12)


NahR_L154F constitutive transcription factor binds upstream of Psal

Codes for a mutant version of NahR transcription factor that binds upstream of Psal promoter. Wild Type version requires salicylate to induce Psal. This allele can induce Psal in the absence of salicylate (J Biol Chem. 1991 Jun 15;266(17):10830-8.). This part is useful to create a positive feedback loop when place downstream of Psal.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 781
    Illegal SapI site found at 1710


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Categories
Parameters
None