Composite

Part:BBa_K2142007

Designed by: Lukas Rieder   Group: iGEM16_NAWI-Graz   (2016-10-07)


Composite part containing ccdA and blue chromoprotein

This part was used to make our E.colis resistant against the ccdB toxin they should produce. For this reason the expressed the (BBa_K2142006). Further we used Anderson promoters and RBS to be sure our proteins are expressed constitutive. This was very important for the reason the Antitoxin has a real short half-life and the chromoprotein was used to detect the winner. At the end of the part we used a bidirectional double terminator, which also terminated the transcription of BBa_K2142005. we demonstrated that or part works. For the results visit http://2016.igem.org/Team:NAWI-Graz/Results

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None