Part:BBa_K2142000

Composite part containing mazE and red chromoprotein

This part was used to make our E.colis resistant against the mazF toxin they should produce. For this reason the expressed the mazE antitoxin (BBa_K2142001). Further we used Anderson promoters and RBS to be sure our proteins are expressed constitutive. This was very important for the reason the Antitoxin has a real short half-life and the chromoprotein was used to detect the winner. At the end of the part we used a bidirectional double terminator, which also terminated the transcription of BBa_K2142005.

We saw that E.coli needs about 16 hours to express the red protein. (Figure1). To see if the other proteins are formed we used SDS-PAGE for the results visit http://2016.igem.org/Team:NAWI-Graz/Results

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 815
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]