Coding

Part:BBa_K2141002

Designed by: Altug Uludag, Nedim Haciosmanoglu, Galip Can Guclu, Yagmur Guneri, Melis Akgun   Group: iGEM16_Istanbul_Tech   (2016-10-14)

eCFP-antiMeth scFv-eYFP

This part is designed for detection of methamphetamine by using meth-binding scFv antibody, which binds with methamphetamine. Two fluorescent proteins, enhanced CFP and enhanced YFP, present in our design to obtain FRET signal when the absence of methamphetamine, and give FRET signals. When the presence of methamphetamine, two domains of the anti-meth scFv binds together, keeps the fluorescent proteins far from each other, thus blocks the FRET signal. Also our construct includes a linker to keep whole construct together.


ECFP-meth scFv-EYFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1096
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 936
  • 1000
    COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None