Project

Part:BBa_K2140005

Designed by: Robert Ashley Naturani   Group: iGEM16_Melbourne   (2016-10-13)


StarScafold NonSequential 1

Sequential intein activation is not required for enzyme splicing, we have a set of four previously tested inteins we can use for enzyme co-localisation which have been shown to have a high splicing rate, yield and orthologous splicing reactions. This part contains the coding sequence for our Non-Sequential StarScaffold, which allows us to check structural changes that occur due to splicing reactions occurring in the same mixture but at alternate temperatures.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 173
  • 1000
    COMPATIBLE WITH RFC[1000]


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