Composite

Part:BBa_K2139014

Designed by: UBC iGEM 2016   Group: iGEM16_British_Columbia   (2016-10-14)


g12 with Terminator

This part encompass the coding region for an endo-beta-1,4-glucanase known as G12 and a double terminator. G12 catalyzes the conversion of cellulose into smaller fragments by making internal cuts in the cellulose chain. It can be used in conjunction with exoglucanases and beta-1,4-glucosidases for the direct conversion of cellulose into monomeric glucose. The protein is monomeric with an optimal temperature of 50 degrees Celsius and an optimal pH ranging from 6 to 8. The genbank ascension number for this protein is ABK52392.1 (amino acid) and a PDB code of 1H0B_A (homolog).

Literature data for this part can be found http://aem.asm.org/content/76/19/6360.full

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 777


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Parameters
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